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Xenovea offers NGS-based Methyl-Seq services to detect DNA methylation across the entire genome, including CpG and less common CHG and CHH contexts.
Whole genome or genome-wide methylation sequencing on NovaSeq 6000 enables you to identify differentially methylated regions, and for discovery of epigenetic biomarkers

Whole Genome Methylation Sequencing (Methyl-Seq)

€100.00Price
Quantity

  • WGBS Specifications

    Sample: ≥20 μl, ≥100 ng/μl High quality DNA

     

    Library preparation: EZ DNA Methylation Lightning MagPrep Kit (Zymo) and NextFlex Rapid XP DNA-Seq kit v2 with UDIs (PerkinElmer)

     

    Inclusions (Sequencing, Bioinformatics, Raw data transfer): WGBS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
    free data transfer of FastQ, Bam files and Reports

     

    Sequencing metrics: Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 120 Gbp per sample; 30x coverage; Q30 ≥ 85%

     

    Quality control: Full quality assurance and guaranteed specifications

     

    Turnaround time: min. 2 – max. 4 weeks

     

    WGEMS Specifications

    Sample: ≥20 μl, ≥10 ng/μl High quality DNA

     

    Library preparation: NEBNext Enzymatic Methyl-seq library preparation system from New England Biolabs (NEB)

     

    Inclusions (Sequencing, Bioinformatics, Raw data transfer): WGEMS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
    free data transfer of FastQ, Bam files and Reports

     

    Sequencing metrics: Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 120 Gbp per sample; 30x coverage; Q30 ≥ 85%

     

    Quality control: Full quality assurance and guaranteed specifications

     

    Turnaround time: min. 2 – max. 4 weeks

     

    RRBS Specifications

    Sample: ≥20 μl, ≥20 ng/μl High quality DNA

     

    Library preparation: Ovation RRBS Methyl-Seq system (NuGEN)

     

    Inclusions (Sequencing, Bioinformatics, Raw data transfer): RRBS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation and variant reports);
    free data transfer of FastQ, Bam files and Reports

     

    Sequencing metrics: Performed on Illumina NovaSeq 6000 with 3 Gbp throughput per sample; Q30 ≥ 85%

     

    Quality control: Full quality assurance and guaranteed specifications

     

    Turnaround time: min. 2 – max. 4 weeks

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