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Targeting poly(A) RNAs provides a sensitive option for detection and quantitation of even rare coding transcript variants

mRNA-Seq

PriceFrom €95.00
Quantity

  • Sample: Tissue/biosample on dry ice or ≥20 μl, ≥20 ng/μl High quality RNA (RIN>7)

     

    Library preparation: Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)

     

    Inclusions: mRNA-Seq analysis includes sample quality control, library preparation, final QC

     

    Sequencing metrics: Performed on Illumina NovaSeq with 2x150 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 85% 

     

    Basic Bioinfromatics: Filtering, trimming

     

    Raw data transfer: Free data transfer of FastQ files

     

    Quality control: Full quality assurance and guaranteed specifications

     

    Turnaround time: 4-5 weeks

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