Gene Expression Profiling (GEx)

Sample: Tissue/biosample on dry ice or ≥20 µl, ≥50 ng/µl High or Low quality RNA

 

Library preparation: QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)

 

Inclusions: GEx analysis includes RNA quantitation/qualification (QC), library preparation, sequencing

 

Sequencing metrics: Performed on Illumina NovaSeq with ≥75 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 85%

 

Basic bioinformatic analysis: demultiplexing, filtering, trimming

 

Raw data transfer: free data transfer of FastQ files

 

Quality control: Full quality assurance and guaranteed specifications

 

Turnaround time: 4-6 weeks